Ukr.Biochem.J. 2018; Том 90, № 4, липень-серпень, c. 90-101

doi: https://doi.org/10.15407/ubj90.04.090

Нітрати і нітрити у питній воді впливають на антиоксидантні ензими в еритроцитах щурів

26.09.2018   Uncategorized   No comments

E. Sierra-Campos1, M. A. Valdez-Solana1, M. I. Campos-Almazán1, C. Avitia‑Domínguez2, J. L. Hernández-Rivera1, J. A. de Lira-Sánchez1, G. Garcia-Arenas3, A. Téllez-Valencia2

1Faculty of Chemical Sciences, Campus Gómez Palacio, University Juarez of Durango State, Gomez Palacio, Durango, Mexico;
e-mail: ericksier@gmail.com;
2Faculty of Medicine and Nutrition, Campus Durango, University Juarez of Durango State, Durango, Mexico;
3Faculty of Health Sciences, Campus Gómez Palacio, University Juarez of Durango State, Gomez Palacio, Durango, Mexico

У дослідженні оцінювали вплив короткострокового споживання питної води, що містить нітрати і нітрити, на антиоксидантну систему і мембрани еритроцитів щурів. Щури Wistar були випадковим чином розділені на три групи: тварини 1-ї групи (контрольної) пили тільки дистильовану воду ad libitum; у 2-й групі – воду, що містила нітрати в дозі 124 мг/кг; а в 3-й групі – воду, що містила нітрити в дозі 150 мг/кг маси тіла протягом 7 днів. Наприкінці експерименту в щурів 3-ї групи спостерігали значне зниження активності глутатіонпероксидази (GPx), глюкозо-6-фосфатдегідрогенази (G6PDH) і каталази (CAT); у щурів 2-ї групи активність GPx і CAT була значно знижена, тоді як активність глутатіонредуктази і рівень пероксинітриту були без змін. Рівень малондіальдегіду (MDA) був збільшений в обох групах порівняно з контрольною групою. Також показано, що рівень метгемоглобіну і осмотична крихкість змінювалися в щурів, які отримували воду з нітратами та нітритами, порівняно з контрольною групою. Зміни були найвираженішими в щурів 3-ї групи. Дійшли висновку, що нітрати і нітрити впливають на стан антиоксидантної системи в еритроцитах щурів, в основному, за рахунок зміни активності NADPH ензимів.

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