Ukr.Biochem.J. 2022; Том 94, № 4, липень-серпень, c. 67-82
doi: https://doi.org/10.15407/ubj94.04.067
Розподіл і поліморфізм ензимів антиоксидантного захисту та біотрансформації ксенобіотиків у середземноморської мідії Mytilus galloprovincialis
В. А. Топтіков, І. Ю. Чубик*, С. В. Чеботар
Одеський національний університет імені І. І. Мечникова, Україна;
*e-mail: i.chubyk@onu.edu.ua
Отримано: 18 липня 2022; Виправлено: 17 жовтня 2022;
Затверджено: 04 листопада 2022; Доступно онлайн: 14 листопада 2022
Дослідження полягало у визначенні тканинного розподілу, активності і поліморфізму 13 ензимів, що забезпечують антиоксидантний захист та біотрансформацію ксенобіотиків у п’яти органах двостулкових молюсків (гепатопанкреас, ктенідії, мантія, нога, мускул-аддуктор). Ізо-алозимний аналіз проводили методом електрофорезу, кореляційні зв’язки між досліджуваними ензимами у тілі мідії здійснювали за допомогою кореляційного аналізу методом Спірмена. Встановлено, що всі досліджувані ензими є поліморфними, кожен орган відрізняється рівнем активності ензимів та набором множинних форм. Показано скоординоване функціонування захисних ензимних систем у різних органах мідій.
Ключові слова: Mytilus galloprovincialis, ізо-алозимний аналіз, антиоксидантні ензими, ензими біотрансформації, скоординованість захисних ензимних систем
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