Ukr.Biochem.J. 2023; Том 95, № 1, січень-лютий, c. 5-19
doi: https://doi.org/10.15407/ubj95.01.005
CRISPR як нова технологія для діагностики COVID-19
A. A. Saleem1*, A. F. Annooz2, I. M. A. Hadi3, A. H. H. Kabla4
1Medical Laboratory Techniques, Al-Hakim General Hospital, Najaf, Iraq;
2Faculty of Medicine, University of Kufa, Najaf, Iraq;
3Medical Laboratory Techniques, Al-Sadr Medical city, Najaf, Iraq;
4National Advanced IPv6 Centre, Universiti Sains Malaysia, Penang, Malaysia;
*e-mail: aliadil41994@gmail.com
Отримано: 30 листопада 2022; Виправлено: 21 лютого 2023;
Затверджено: 13 квітня 2023; Доступно онлайн: 27 квітня 2023
До теперішнього часу захворювання людини на COVID-19, що виникає внаслідок тяжкого гострого респіраторного синдрому, спричиненого коронавірусом 2 (SARS-CoV-2), залишається гострою проблемою для усього світу. У січні 2022 року зареєстровано понад 300 мільйонів випадків інфікування та понад 5 мільйонів смертей від COVID-19. Щоб протистояти такому швидкому поширенню, вкрай необхідні ефективні та недорогі методи діагностики, які допоможуть контролювати небезпеку пандемії. Технологія CRISPR довела свою ефективність у виявленні COVID-19 завдяки своїй простоті, специфічності та високій чутливості. У цьому огляді розглянуто сучасний стан розробки платформ CRISPR для діагностики та лікування COVID-19. Обговорюються обмеження та можливості CRISPR з точки зору методів аналізу нуклеїнових кислот для діагностики вірусних інфекцій.
Ключові слова: COVID-19, CRISPR, RT-PCR, SARS-COV-2, протеїн Cas
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