Ukr.Biochem.J. 2018; Том 90, № 3, травень-червень, c. 17-31

doi: https://doi.org/10.15407/ubj90.03.017

Аналіз c-di-GMP і можливого почуття кворуму в Pseudomonas fluorescens SBW25: зв’язок між внутрішньо- і міжклітинною регуляцією сприяє кооперативній поведінці в угрупованні і формуванню біоплівки

О. В. Мошинець1, Д. Фостер2, С. О. Карахім3, К. МакЛохлін4, С. П. Рогальський5, С. Ю. Римар1, Г. П. Волинець1, Е. Д. Спайрс4

1Інститут молекулярної біології і генетики НАН України, Київ;
e-mail: moshynets@gmail.com;  e-mail: galina.volinetc@gmail.com
2Оксфордcький університет, Госпіталь ім. Джона Редкліфа, Велика Британія;
e-mail: dona.foster@ndm.ox.ac.uk;
3Інститут біохімії ім. О. В. Палладіна, НАН України, Київ;
e-mail: laserlab@biochem.kiev.ua;
4Школа науки, інжинірингу і технологій, Абертейський університет, Велика Британія;
e-mail: mclaughlinkimberley@gmail.com; e-mail: a.spiers@abertay.ac.uk;
5Інститут біоорганічної хімії і нафтохімії НАН України, Київ;
e-mail: sergey.rogalsky@gmail.com

Успішність бактеріальної колонізації складних еконіш вимагає індивідуальної відповіді на зміни умов на мікрорівні так само як і кооперацію на рівні угруповання для продукції таких великомасштабних структур як біоплівки. Координація індивідуальних відповідей і відповідей угруповання може бути досягнута шляхом зв’язування внутрішньоклітинних сенсорних і регуляторних систем, опосередкованих біс-(3′,5′)-циклічним димерним гуанозинмонофосфатом (c-di-GMP) та іншими сполуками індивідуумів із міжклітинною регуляцією – почуттям кворуму (ПК), що контролює популяцію. Накопичується все більше доказів того, що формування біоплівки багатьма псевдомонадами регулюється як внутрішньоклітинними, так і міжклітинними регуляторними системами, хоча в разі модельної Pseudomonas fluorescens SBW25 Wrinkly Spreader, у котрій мутації, що підвищують рівні c-di-GMP, призводять до створення міцної целюлозної біоплівки на кордоні розділу фаз повітря–рідина, не було виявлено ніякого свідоцтва залучення кворумзалежної регуляції. Проте наш недавній огляд геному P. fluorescens SBW25 виявив потенційний ПК-залежний регуляторний шлях та інші ПК-залежні гени, пов’язані з гомеостазом c-di-GMP, а молекули ПК-сигналінгу було ідентифіковано в культурі. Ці дані свідчать про можливий зв’язок між c-di-GMP-регуляцією і ПК у P. fluorescens SBW25, що дозволяє проводити більш складний і гнучкий контроль продукції целюлози і утворення біоплівки за колонізації ґрунтів і еконіш, асоційованих з рослинами, – природними середовищами існування P. fluorescens SBW25.

Ключові слова: , , , , ,


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