Ukr.Biochem.J. 2019; Том 91, № 4, липень-серпень, c. 33-40

doi: https://doi.org/10.15407/ubj91.04.033

Джерела і закономірності синтезу оксиду азоту в клітинах гладенького м’яза матки

26.07.2019   Uncategorized   No comments

Г. В. Данилович, Ю. В. Данилович, Т. В. Богач,
В. Т. Гурська, С. О. Костерін

Інститут біохімії ім. О. В. Палладіна НАН України, Київ;
e-mail: danylovych@biochem.kiev.ua

Отримано: 28 лютого 2019; Затверджено: 17 травня 2019

Доведено, що синтез NO в ізольованих мітохондріях гладенького м’яза матки щурів є залежним від надходження екзогенного Са2+ до мітохондрій (інгібується 1–10 мМ Mg2+ за відсутності АТР, 10 мкМ рутенієвим червоним), пригнічується антагоністами кальмодуліну (0,1–10 мкМ кальмідозоліумом та 1–100 мкМ трифлуоперазином). Він блокується відомим антагоністом конститутивних NO-синтаз NG-нітро-L-аргініном з ефектом полумаксимального інгібування близько 25 мкМ. Помірна дехолестеринізація плазматичної мембрани міоцитів за їх обробки 0,01%-им дигітоніном приводить до зростання синтезу NO клітинами, що свідчить на користь того, що плазмалема є можливим джерелом NO в міоцитах матки.

Ключові слова: , , , ,

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