Ukr.Biochem.J. 2015; Том 87, № 1, січень-лютий, c. 33-45
doi: http://dx.doi.org/10.15407/ubj87.01.033
Сучасні флуоресцентні методи дослідження механізмів активації лімфоцитів
Г. А. Любченко1, Р. М. Морєв2, Л. С. Холодна1
1Київський нацiональний унiверситет iмені Тараса Шевченка, Україна;
2Інститут фізіології ім. О. О. Богомольця НАН України, Київ;
е-mail: gannalb@yahoo.com
Флуоресцентні протеїни є найперспективнішими інструментами для вивчення сигнальних процесів у лімфоцитах. Цей короткий огляд підсумовує нові досягнення в отриманнi зображень на основі методів флуоресценції, що розвивалися останніми роками, також обговорюються нові технологічні та методологічні досягнення, такі як люмінесцентнi фотоперемикачі, відновлення флуоресценції після фотознебарвлення (FRAP), перенесення енергії за допомогою флуоресцентного резонансу (FRET), флуоресцентна мікроскопія зображення тривалості життя (FLIM), фотоактивована локалізаційна мікроскопія (PALM), стохастична оптична мікроскопія реконструкції (STORM), стимульоване виснаження, індуковане випромінюванням (STED), повне внутрішнє відбиття флуоресценції (TIRF) та інші методики. Цей огляд також висвітлює останні досягнення в області аналізу зображень живих тканин in vitro, нові застосування проточної цитофлуориметрії iз використанням генетично кодованих флуоресцентних протеїнів і перспективи для розвитку нових імунологічних тест-систем на основі технології флуоресцентних протеїнів, сучасні прикладні підходи до використання проточної цитометрії з генетично кодованими флуоресцентними протеїнами, а також перспективи розвитку нових імунологічних тест-систем на основі технології флуоресцентних протеїнів.
Ключові слова: активація лімфоцитів, тест-системи, флуоресцентні методи, флуоресцентні протеїни
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