Ukr.Biochem.J. 2015; Том 87, № 6, листопад-грудень, c. 129-135

doi: http://dx.doi.org/10.15407/ubj87.06.129

Індукована алюмінієвою токсичністю відповідь фенольного метаболізму в рослинах Fagopyrum esculentum Moench.

О. Є. Смірнов, А. М. Косян, О. І. Косик, Н. Ю. Таран

ННЦ «Інститут біології», Київський національний університет імені Тараса Шевченка, Україна;
e-mail: plantaphys@gmail.com

Рід гречка (Fagopyrum Mill.) – один з алюморезистентних рослинних таксонів. Метою дослідження було з’ясувати вплив йонів алюмінію (50 мкМ) на накопичення речовин фенольної природи в різних час­тинах рослин гречки­ звичайної (Fagopyrum esculentum Moench.). Показано підвищення вмісту загальної суми фенольних сполук, зміни у вмісті флавоноїдів та антоціанів та активності фенілаланін аміак-ліази (ФАЛ) на десяту добу експозиції рослин у присутності алюмінію. Найбільші зміни відмічено в тканинах досліджуваних листків – збільшення вмісту загальної суми фенольних сполук на 27,2% та підвищення активності ФАЛ у 2,5 раза. При цьому спостерігали інгібування активності ензиму в тканинах коренів. Одержані дані можуть бути корисними для розуміння принципів алюморезистентності гречки й участі фенольних сполук у механізмах адаптації.

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Посилання:

  1. Feng Ma J, Hiradate S, Matsumoto H. High aluminum resistance in buckwheat. II. Oxalic acid detoxifies aluminum internally . Plant Physiol. 1998 Jul;117(3):753-9. PubMed, PubMedCentral, CrossRef
  2. Shen R, Ma JF. Distribution and mobility of aluminium in an Al-accumulating plant, Fagopyrum esculentum Moench. J Exp Bot. 2001 Aug;52(361):1683-7. PubMed
  3. Ma JF. Role of organic acids in detoxification of aluminum in higher plants. Plant Cell Physiol. 2000 Apr;41(4):383-90. Review. PubMed, CrossRef
  4.  Yamamoto Y, Kobayashi Y, Devi SR, Rikiishi S, Matsumoto H. Aluminum toxicity is associated with mitochondrial dysfunction and the production of reactive oxygen species in plant cells. Plant Physiol. 2002 Jan;128(1):63-72. PubMed, PubMedCentral, CrossRef
  5. Choudhury S, Panda P, Sahoo L, Panda SK. Reactive oxygen species signaling in plants under abiotic stress. Plant Signal Behav. 2013 Apr;8(4):e23681. Review. PubMed, CrossRef
  6. Fan QJ, Liu JH. Nitric oxide is involved in dehydration/drought tolerance in Poncirus trifoliata seedlings through regulation of antioxidant systems and stomatal response. Plant Cell Rep. 2012 Jan;31(1):145-54. PubMed, CrossRef
  7. Ferdinando M, Brunetti C, Fini A, Tattini M. Flavonoids as antioxidants in plants under abiotic stresses. In: Ahmad P, Prasad MNV (eds) Abiotic Stress Responses in Plants: metabolism, productivity and sustainability. Springer Science+Business Media, LLC, New York, 2012; P. 159-179.
  8. Apel K, Hirt H. Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annu Rev Plant Biol. 2004;55:373-99. Review. PubMed, CrossRef
  9. Koukol J, Conn EE. The metabolism of aromatic compounds in higher plants. IV. Purification and properties of the phenylalanine deaminase of Hordeum vulgare. J Biol Chem. 1961 Oct;236:2692-8. PubMed
  10. Boudet AM. Evolution and current status of research in phenolic compounds. Phytochemistry. 2007 Nov-Dec;68(22-24):2722-35. PubMed, CrossRef
  11. Dixon RA, Paiva NL. Stress-induced phenylpropanoid metabolism. Plant Cell. 1995 Jul;7(7):1085-1097. PubMed, PubMedCentral, CrossRef
  12.  Bobo-García G, Davidov-Pardo G, Arroqui C, Vírseda P, Marín-Arroyo MR, Navarro M. Intra-laboratory validation of microplate methods for total phenolic content and antioxidant activity on polyphenolic extracts, and comparison with conventional spectrophotometric methods. J Sci Food Agric. 2015 Jan;95(1):204-9. PubMed, CrossRef
  13.  Li X, Kim JK, Park SY, Zhao S, Kim YB, Lee S, Park SU. Comparative analysis of flavonoids and polar metabolite profiling of Tanno-original and Tanno-high rutin buckwheat. J Agric Food Chem. 2014 Mar 26;62(12):2701-8. PubMed, CrossRef
  14. Petry RD, Ortega GG, Silva WB. Flavonoid content assay: influence of the reagent concentration and reaction time on the spectrophotometric behavior of the aluminium chloride–flavonoid complex.  Pharmazie. 2001 Jun;56(6):465-70. PubMed
  15. Jaleel CA, Wang G, Ahmad P. Changes in the photosynthetic characteristics of Catharanthus roseus L. as a result of exogenous growth regulators. Plant Omics J. 2009;2(4):169-174.
  16. Giusti M. M., Wrolstad R. E. Unit F1.2: anthocyanins. Characterization and measurement with UV-visible spectroscopy. In: Wrolstad RE (ed) Current protocols in food analytical chemistry. John Wiley & Sons, New York, 2001; pp. F1.2.1–1.2.13.
  17. Zucker M. Induction of Phenylalanine Deaminase by Light and its Relation to Chlorogenic Acid Synthesis in Potato Tuber Tissue. Plant Physiol. 1965 Sep;40(5):779-84. PubMed, PubMedCentral, CrossRef
  18. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248-54. PubMed, CrossRef
  19.  Pereira P., Cambraia J., Sant’Anna R., Mosquim P. R., Moreira M. A. Aluminium effects on lipid peroxidation and the activities of enzymes of oxidative metabolism in sorghum. Rev Bras Plant Nutr. 1999; 43: 1009-1014.
  20. Yin R, Messner B, Faus-Kessler T, Hoffmann T, Schwab W, Hajirezaei MR, von Saint Paul V, Heller W, Schäffner AR. Feedback inhibition of the general phenylpropanoid and flavonol biosynthetic pathways upon a compromised flavonol-3-O-glycosylation. J Exp Bot. 2012 Apr;63(7):2465-78. PubMed, PubMedCentral, CrossRef
  21. Kärkönen A, Koutaniemi S, Mustonen M, Syrjänen K, Brunow G, Kilpeläinen I, Teeri TH, Simola LK. Lignification related enzymes in Picea abies suspension cultures. Physiol Plant. 2002 Mar;114(3):343-353. PubMed, CrossRef
  22. Michalak A. Phenolic compounds and their antioxidant activity in plants growing under heavy metal stress. Polish J Environ Stud. 2006; 15(4): 523-530.
  23. Hossain MA, Zakir Hossain AKM, Kihara T, Koyama H, Hara T. Aluminum-induced lipid peroxidation and lignin deposition are associated with an increase in H2O2 generation in wheat seedlings. Soil Sci Plant Nutr. 2005; 51(2): 223-230.  CrossRef
  24.   Sujkowska-Rybkowska M, Borucki W. Localization of hydrogen peroxide accumulation and diamine oxidase activity in pea root nodules under aluminum stress. Micron. 2014 Feb;57:13-22.  PubMed, CrossRef
  25. Boscolo PR, Menossi M, Jorge RA. Aluminum-induced oxidative stress in maize. Phytochemistry. 2003 Jan;62(2):181-9. PubMed, CrossRef
  26.  Babourina O, Ozturk L, Cakmak I, Rengel Z. Reactive oxygen species production in wheat roots is not linked with changes in H+ fluxes during acidic and aluminium stresses. Plant Signal Behav. 2006 Mar;1(2):71-6. PubMed, PubMedCentral, CrossRef
  27. Schützendübel A, Polle A. Plant responses to abiotic stresses: heavy metal-induced oxidative stress and protection by mycorrhization. J Exp Bot. 2002 May;53(372):1351-65. Review. PubMed, CrossRef
  28. Winkel-Shirley B. Biosynthesis of flavonoids and effects of stress. Curr Opin Plant Biol. 2002 Jun;5(3):218-23. Review. PubMed, CrossRef
  29. Diaz J, Bernal A, Pomar F, Merino F. Induction of shikimate dehydrogenase and peroxidase in pepper (Capsicum annuum L.) seedlings in response to copper stress and its relation to lignification. Plant Sci. 2001 Jun;161(1):179-188. CrossRef
  30. Saha D, Mandal S, Saha A. Copper induced oxidative stress in tea (Camellia sinensis) leaves. J Environ Biol. 2012 Sep;33(5):861-6. PubMed
  31. Procházková D, Boušová I, Wilhelmová N. Antioxidant and prooxidant properties of flavonoids. Fitoterapia. 2011 Jun;82(4):513-23. Review. PubMed, CrossRef
  32. Sakihama Y, Cohen MF, Grace SC, Yamasaki H. Plant phenolic antioxidant and prooxidant activities: phenolics-induced oxidative damage mediated by metals in plants. Toxicology. 2002 Aug 1;177(1):67-80. Review. PubMed, CrossRef
  33. Bayrakçeken F, Aktaş S, Toptan M, Unlügedik A. High resolution electronic absorption spectra of anisole and phenoxyl radical. Spectrochim Acta A Mol Biomol Spectrosc. 2003 Jan 1;59(1):135-8. PubMed, CrossRef
  34. Cohen MF, Sakihama Y, Yamasaki H. Roles of plant flavonoids in interactions with microbes: from protection against pathogens to the mediation of mutualism. In: Pandalai SG (ed.) Recent Research Developments in Plant Physiology 2. Research Signpost, Trivandrum, 2001; P. 157-173.
  35. Kalyanaraman B. Characterization of o-semiquinone radicals in biological systems. Methods Enzymol. 1990;186:333-43. PubMed, CrossRef

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