Ukr.Biochem.J. 2019; Том 91, № 1, січень-лютий, c. 5-20

doi: https://doi.org/10.15407/ubj91.01.005

Ліпополісахариди Рantoea agglomerans: структура, функціональна та біологічна активність

10.04.2019   Uncategorized   No comments

Л. Д. Варбанець, Т. В. Булигіна, Л. А. Пасічник, Н. В. Житкевич

Інститут мікробіології і вірусології ім. Д. К. Заболотного НАН України, Київ;
e-mail: varbanets_imv@ukr.net

В огляді наведені дані літератури, а також результати власних експериментальних досліджень ліпополісахаридів (ЛПС) грамнегативних бактерій. Основну увагу автори приділяють Рantoea agglomerans, представнику родини Enterobacteriaceae. Вперше описано унікальні структури О-специфічних полісахаридних ланцюгів їхніх ліпополісахаридів, які можуть бути як розгалу­женими, так і лінійними тетра- і пентасахаридними ланцюгами, що повторюються. Показана гетерогенність як самої молекули ЛПС, так і присутність в бактеріальній клітині декількох ЛПС, які відрізняються структурою ліпідів А, О-специфічних полісахаридних ланцюгів, серологічною активністю, а також  ендотоксичними властивостями, зокрема токсичністю і пірогенністю. Така гетерогенність є одним із механізмів поліфункціональності ЛПС. На основі О-антигенності ЛПС вперше було проведено серотипування штамів Р. agglomerans і віднесено їх до 10 серогруп. Висока імуномодулювальна  активність ЛПС Р. agglomerans дозволяє припустити використання їхніх олігосахаридних фрагментів для створення кон’югованих вакцин проти захворювань, спричинених грамнегативними бактеріями.

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