Ukr.Biochem.J. 2015; Том 87, № 1, січень-лютий, c. 91-98

doi: http://dx.doi.org/10.15407/ubj87.01.091

Фулерен C(60) запобігає генотоксичній дії доксорубіцину на лімфоцити людини in vitro

К. С. Афанасьєва1, С. В. Прилуцька1, О. В. Лозовик1, К. І. Богуцька1,
А. В. Сиволоб1, Ю. І. Прилуцький1, У. Ріттер2, П. Шарфф2

1Київський національний університет імені Тараса Шевченка, Україна;
е-mail: prylut@ukr.net;
2Технічний університет Ілменау, Інститут хімії і біотехнології, Ілменау, ФРН

Самоорганізація фулерену C60, доксо­рубіцину та їх суміші, осаджених із водних розчинів, була досліджена з використанням методу атомно-силової мікроскопії. Результати передбачають комплексоутворення між цими двома препаратами. Генотоксичність комплексу фулерену C60 із доксорубіцином (С60+Докс) оцінювали in vitro на лімфоцитах людини за допомогою методу ДНК-комет. Одержані результати демонструють, що фулерен C60 запобігає токсичній дії Докс на нормальні клітини і, відповідно, комплекс С60+Докс може бути використаний у біомедичних цілях.

Ключові слова: , , , ,


Посилання:

  1. Anilkumar P, Lu F, Cao L, Luo PG, Liu J H, Sahu S, Tackett KN, Wang Y, Sun YP. Fullerenes for Applications in Biology and Medicine. Curr Med Chem. 2011;18 (14):2045-2059. CrossRef
  2. Medicinal chemistry and pharmacological potential of fullerenes and carbon nanotubes. Series: Carbon materials: chemistry and physics. Cataldo F, Da Ros T. (eds.). Netherlands: Springer, 2008;1:408 р.
  3. Fullerenes. Principles and applications. eds.: Langa de la Puente F., Nierengarten J. F. Cambridge: RSC Edition, 2007. 400 p.
  4. Andrievsky G, Klochkov V, Derevyanchenko L. Is C60 fullerene molecule toxic?! Fullerenes, Nanotubes, Carbon Nanostruct. 2005;13:363-376. CrossRef
  5. Ema M, Tanaka J, Kobayashi N, Naya M, Endoh S, Maru J, Hosoi M, Nagai M, Nakajima M, Hayashi M, Nakanishi J. Genotoxicity evaluation of fullerene C60 nanoparticles in a comet assay using lung cells of intratracheally instilled rats. Regul Toxicol Pharmacol. 2012 Apr;62(3):419-24. PubMed, CrossRef
  6. Folkmann JK, Risom L, Jacobsen NR, Wallin H, Loft S, Møller P. Oxidatively damaged DNA in rats exposed by oral gavage to C60 fullerenes and single-walled carbon nanotubes. Environ Health Perspect. 2009 May;117(5):703-8. PubMed, PubMedCentral, CrossRef
  7. Matsuda S, Matsui S, Shimizu Y, Matsuda T. Genotoxicity of colloidal fullerene C60. Environ Sci Technol. 2011 May 1;45(9):4133-8. PubMed, CrossRef
  8. Wallin H, Jacobsen NR, White PA, Gingerich J, Møller P, Loft S, Vogel U. Mutagenicity of carbon nanomaterials. J Biomed Nanotechnol. 2011 Feb;7(1):29. PubMed, CrossRef
  9. Dhawan A, Taurozzi JS, Pandey AK, Shan W, Miller SM, Hashsham SA, Tarabara VV. Stable colloidal dispersions of C60 fullerenes in water: evidence for genotoxicity. Environ Sci Technol. 2006 Dec 1;40(23):7394-401. PubMed, CrossRef
  10. Jacobsen NR, Pojana G, White P, Møller P, Cohn CA, Korsholm KS, Vogel U, Marcomini A, Loft S, Wallin H. Genotoxicity, cytotoxicity, and reactive oxygen species induced by single-walled carbon nanotubes and C(60) fullerenes in the FE1-Mutatrade markMouse lung epithelial cells. Environ Mol Mutagen. 2008 Jul;49(6):476-87. PubMed, CrossRef
  11. Al-Subiai SN, Arlt VM, Frickers PE, Readman JW, Stolpe B, Lead JR, Moody AJ, Jha AN. Merging nano-genotoxicology with eco-genotoxicology: an integrated approach to determine interactive genotoxic and sub-lethal toxic effects of C60 fullerenes and fluoranthene in marine mussels, Mytilus sp. Mut Res Genetic Toxicol Environ Mutagen. 2012;745(1-2):92-103. CrossRef
  12. Totsuka Y, Higuchi T, Imai T, Nishikawa A, Nohmi T, Kato T, Masuda S, Kinae N, Hiyoshi K, Ogo S, Kawanishi M, Yagi T, Ichinose T, Fukumori N, Watanabe M, Sugimura T, Wakabayashi K. Genotoxicity of nano/microparticles in in vitro micronuclei, in vivo comet and mutation assay systems. Part Fibre Toxicol. 2009 Sep 3;6:23. PubMed, PubMedCentral, CrossRef
  13. Rouse JG, Yang J, Barron AR, Monteiro-Riviere NA. Fullerene-based amino acid nanoparticle interactions with human epidermal keratinocytes. Toxicol In Vitro. 2006 Dec;20(8):1313-20. PubMed, CrossRef
  14. Shinohara N, Matsumoto K, Endoh S, Maru J, Nakanishi J. In vitro and in vivo genotoxicity tests on fullerene C60 nanoparticles. Toxicol Lett. 2009 Dec 15;191(2-3):289-96. PubMed, CrossRef
  15. Johnston HJ, Hutchison GR, Christen­sen FM, Aschberger K, Stone V. The biological mechanisms and physicochemical characteristics responsible for driving fullerene toxicity. Toxicol Sci. 2010;114(2):162-182. CrossRef
  16. Aschberger K, Johnston HJ, Stone V, Aitken RJ, Tran CL, Hankin SM, Peters SA, Christensen FM. Review of fullerene toxicity and exposure–appraisal of a human health risk assessment, based on open literature. Regul Toxicol Pharmacol. 2010 Dec;58(3):455-73. Review. PubMed, CrossRef
  17. Su Y, Xu JY, Shen P, Li J, Wang L, Li Q, Li W, Xu GT, Fan C, Huang Q. Cellular uptake and cytotoxic evaluation of fullerenol in different cell lines. Toxicology. 2010 Mar 10;269(2-3):155-9. PubMed, CrossRef
  18. Prylutska SV, Grynyuk II, Matyshevska OP, Prylutskyy YuI, Ritter U, Scharff P. Anti-oxidant properties of C60 fullerenes in vitro. Fullerenes, Nanotubes, Carbon Nanostruct. 2008;16(5-6):698-705. CrossRef
  19. Prylutska SV, Grynyuk II, Grebinyk SM, Matyshevska OP, Prylutskyy YI, Ritter U, Siegmund C, Scharff P. Comparative study of biological action of fullerenes C60 and carbon nanotubes in thymus cells. Mat. wiss.Werkstofftech. 2009;40:238-241. CrossRef
  20. Foley S, Crowley C, Smaihi M, Bonfils C, Erlanger BF, Seta P, Larroque C. Cellular localisation of a water-soluble fullerene derivative. Biochem Biophys Res Commun. 2002 May 31;294(1):116-9. PubMed, CrossRef
  21. Prylutska S, Bilyy R, Overchuk M, Bychko A, Andreichenko K, Stoika R, Rybalchenko V, Prylutskyy Y, Tsierkezos NG, Ritter U. Water-soluble pristine fullerenes C60 increase the specific conductivity and capacity of lipid model membrane and form the channels in cellular plasma membrane. J Biomed Nanotechnol. 2012 Jun;8(3):522-7. PubMed, CrossRef
  22. Prylutska SV, Burlaka AP, Klymenko PP, Grynyuk II, Prylutskyy YI, Schütze Ch, Ritte U. Using watersoluble C60 fullerenes in anticancer therapy. Cancer Nanotechnol. 2011;2(1):105-110. CrossRef
  23. Prylutska SV, Burlaka AP, Prylutskyy YI, Ritter U, Scharff P. Pristine C(60) fullerenes inhibit the rate of tumor growth and metastasis. Exp Oncol. 2011 Sep;33(3):162-4. PubMed
  24. Hrelia S, Fiorentini D, Maraldi T, Angeloni C, Bordoni A, Biagi PL, Hakim G. Doxorubicin induces early lipid peroxidation associated with changes in glucose transport in cultured cardiomyocytes. Biochim Biophys Acta. 2002 Dec 23;1567(1-2):150-6. PubMed, CrossRef
  25. Evstigneev MP, Buchelnikov AS, Voronin DP, Rubin YV, Belous LF, Prylutskyy YI, Ritter U. Complexation of C60 fullerene with aromatic drugs. Chemphyschem. 2013 Feb 25;14(3):568-78. Epub 2013 Jan 23. PubMed, CrossRef
  26. Liu JH, Cao L, Luo PG, Yang ST, Lu F, Wang H, Meziani MJ, Haque SA, Liu Y, Lacher S, Sun YP. Fullerene-conjugated doxorubicin in cells. ACS Appl Mater Interfaces. 2010 May;2(5):1384-9. PubMed, CrossRef
  27. Prylutskyy YI, Buchelnikov AS, Voronin DP, Kostjukov VV, Ritter U, Parkinson JA, Evstigneev MP. C60 fullerene aggregation in aqueous solution. Phys Chem Chem Phys. 2013 Jun 21;15(23):9351-60. PubMed, CrossRef
  28. Prylutskyy YI, Petrenko VI, Ivankov OI, Kyzyma OA, Bulavin LA, Litsis OO, Evstigneev MP, Cherepanov VV, Naumovets AG, Ritter U. On the origin of C₆₀ fullerene solubility in aqueous solution. Langmuir. 2014 Apr 15;30(14):3967-70. PubMed, CrossRef
  29. Afanasieva K, Chopei M, Zazhytska M, Vikhreva M, Sivolob A. DNA loop domain organization as revealed by single-cell gel electrophoresis. Biochim Biophys Acta. 2013 Dec;1833(12):3237-44. PubMed, CrossRef
  30. Afanasieva K, Zazhytska M, Sivolob A. Kinetics of comet formation in single-cell gel electrophoresis: loops and fragments. Electrophoresis. 2010 Jan;31(3):512-9. PubMed, CrossRef
  31. Tacar O, Sriamornsak P, Dass CR. Doxorubicin: an update on anticancer molecular action, toxicity and novel drug delivery systems. J Pharm Pharmacol. 2013 Feb;65(2):157-70. Review. PubMed, CrossRef
  32. Hozayen WG, Abou Seif H S. Protective effects of rutin and hesperidin against doxorubicin-induced lipodystrophy and cardiotoxicity in albino rats. J. Am. Sci. 2011;7(12):765-775.

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