Ukr.Biochem.J. 2013; Том 85, № 5, вересень-жовтень, c. 114-123

doi: http://dx.doi.org/10.15407/ubj85.05.114

Вплив окисного стресу на рівень експресії генів TGFB1 і HGF у печінці щурів в умовах тривалої шлункової гіпохлоргідрії та за введення мультипробіотика Симбітер

К. О. Дворщенко, О. О. Берник, А. С. Драницина,
С. А. Сенін, Л. І. Остапченко

Київський національний університет імені Тараса Шевченка, Україна;
e-mail: k21037@gmail.com

Досліджено вільнорадикальні процеси в печінці щурів під час тривалої шлункової гіпохлоргідрії, спричиненої омепразолом. Встановлено підвищення інтенсивності окислювальних процесів у тканині печінки: надлишкове утворення супероксидного аніона, пероксиду водню, кількісні зміни функціональних груп ліпідів, зростання вмісту продуктів пероксидного окислення ліпідів, збільшення ксантиноксидазної активності. Показано, що експресія гену Tgfb1 зростає, а експресія гену Hgf не виявляється, що свідчить про можливий розвиток фіброзу печінки. Встановлено, що за введення мультипробіотика «Симбітер® ацидофільний» концентрований щурам із тривалою шлунковою гіпохлоргідрією вищезазначені показники частково відновлюються до контрольних значень, що свідчить про здатність препарату протидіяти розвитку окисних пошкоджень тканини печінки.

Ключові слова: , , , , ,


Посилання:

  1. Ali T, Roberts DN, Tierney WM. Long-term safety concerns with proton pump inhibitors. Am J Med. 2009 Oct;122(10):896-903. Review. PubMed, CrossRef
  2. García Rodríguez LA, Lagergren J, Lindblad M. Gastric acid suppression and risk of oesophageal and gastric adenocarcinoma: a nested case control study in the UK. Gut. 2006 Nov;55(11):1538-44. PubMed, PubMedCentral, CrossRef
  3.  Beales IL. H. pylori-associated hypochlorhydria. Gastroenterology. 1998 Mar;114(3):618-21. PubMed,   CrossRef
  4.  Jensen RT. Consequences of long-term proton pump blockade: insights from studies of patients with gastrinomas. Basic Clin Pharmacol Toxicol. 2006 Jan;98(1):4-19. Review. PubMed, CrossRef
  5. Lahner E, Annibale B. Pernicious anemia: new insights from a gastroenterological point of view. World J Gastroenterol. 2009 Nov 7;15(41):5121-8. Review. PubMed, PubMedCentral, CrossRef
  6. Jianu CS, Fossmark R, Viset T, Qvigstad G, Sørdal O, Mårvik R, Waldum HL. Gastric carcinoids after long-term use of a proton pump inhibitor. Aliment Pharmacol Ther. 2012 Oct;36(7):644-9. PubMed, CrossRef
  7. Kompanets IV, Korotkiy OG, Karpovets TP, Pilipenko SV, Nikolska VV, Ostapchenko LI, Yankovsky DS. Interferon titer and the 2′,5′-oligoadenylate-synthetase activity in rat thymus lymphocytes in conditions of Omeprazol-caused hypergastrinemia. Biopolym Cell. 2013; 29(1):64-69. CrossRef
  8. Williams C, McColl KE. Review article: proton pump inhibitors and bacterial overgrowth. Aliment Pharmacol Ther. 2006 Jan 1;23(1):3-10. Review. PubMed, CrossRef
  9. Wigg AJ, Roberts-Thomson IC, Dymock RB, McCarthy PJ, Grose RH, Cummins AG. The role of small intestinal bacterial overgrowth, intestinal permeability, endotoxaemia, and tumour necrosis factor alpha in the pathogenesis of non-alcoholic steatohepatitis. Gut. 2001 Feb;48(2):206-11. PubMed, PubMedCentral, CrossRef
  10. Loguercio C, De Simone T, Federico A, Terracciano F, Tuccillo C, Di Chicco M, Cartenì M. Gut-liver axis: a new point of attack to treat chronic liver damage? Am J Gastroenterol. 2002 Aug;97(8):2144-6. PubMed, CrossRef
  11. Zhang R, Brennan ML, Shen Z, MacPherson JC, Schmitt D, Molenda CE, Hazen SL. Myeloperoxidase functions as a major enzymatic catalyst for initiation of lipid peroxidation at sites of inflammation. J Biol Chem. 2002 Nov 29;277(48):46116-22. PubMed, CrossRef
  12. Borodina T, Dvorshchenko K, Vakal S, Dranitsina A, Ostapchenko L. Evidence of oxidative stress development in pancreatic cells of rats with chronically suppressed gastric acid secretion. Bull Taras Shevchenko Nat Univ. Kyiv. Series “Biology”.  2013;2(6):27-31.
  13. Lichtman SN, Sartor RB, Keku J, Schwab JH. Hepatic inflammation in rats with experimental small intestinal bacterial overgrowth. Gastroenterology. 1990 Feb;98(2):414-23. PubMed
  14. Gäbele E, Dostert K, Hofmann C, Wiest R, Schölmerich J, Hellerbrand C, Obermeier F. DSS induced colitis increases portal LPS levels and enhances hepatic inflammation and fibrogenesis in experimental NASH. J Hepatol. 2011 Dec;55(6):1391-9.  PubMed, CrossRef
  15. Dooley S, ten Dijke P. TGF-β in progression of liver disease. Cell Tissue Res. 2012 Jan;347(1):245-56. Review. PubMed, PubMedCentral, CrossRef
  16. Giannelli G, Mazzocca A, Fransvea E, Lahn M, Antonaci S. Inhibiting TGF-β signaling in hepatocellular carcinoma. Biochim Biophys Acta. 2011 Apr;1815(2):214-23. Review. PubMed, CrossRef
  17. Gohda E, Nakamura S, Yamamoto I, Minowada J. Hepatocyte growth factor–pleiotropic cytokine produced by human leukemia cells. Leuk Lymphoma. 1995 Oct;19(3-4):197-205. Review. PubMed, CrossRef
  18. Morishita R, Aoki M, Hashiya N, Yamasaki K, Kurinami H, Shimizu S, Makino H, Takesya Y, Azuma J, Ogihara T. Therapeutic angiogenesis using hepatocyte growth factor (HGF). Curr Gene Ther. 2004 Jun;4(2):199-206. Review. PubMed,   CrossRef
  19.  Gratz SW, Mykkanen H, El-Nezami HS. Probiotics and gut health: a special focus on liver diseases. World J Gastroenterol. 2010 Jan 28;16(4):403-10. Review. PubMed, PubMedCentral, CrossRef
  20. Dvorshchenko KO, Vakal SYe, Dranitsiyna AS, Senin SA, Ostapchenko LI. Stress-responsive systems in rat pancreas upon long-term gastric hypochlorhydria and administration of multiprobiotic “Symbiter®”. Ukr Biokhim Zhurn. 2013 Mar-Apr;85(2):68-77. Ukrainian. PubMed, CrossRef
  21. Vakal SE, Dvorshchenko KO, Dranitsina AS, Borodina TV, Ostapchenko LI. Expression of Cckbr, Gast, Reg1α, Tgfb1 genes in rat pancreas upon long-term hypoacidity and with administration of multiprobiotic «Symbiter® acidophilic» concentrated. Biopolym Cell. 2012; 28(6):461-467. CrossRef
  22. Yankovskiy DS., Dyment GS. Multicomponent “Symbiter” probiotics: results and prospects of biodesign and clinical application. Zdorov’e Zhenshchiny. 2006;(3):181-188.
  23. Sutherland MW, Learmonth BA. The tetrazolium dyes MTS and XTT provide new quantitative assays for superoxide and superoxide dismutase. Free Radic Res. 1997 Sep;27(3):283-9. PubMed, CrossRef
  24. Nourooz-Zadeh J, Tajaddini-Sarmadi J, Wolff SP. Measurement of plasma hydroperoxide concentrations by the ferrous oxidation-xylenol orange assay in conjunction with triphenylphosphine. Anal Biochem. 1994 Aug 1;220(2):403-9. PubMed, CrossRef
  25. Hashimoto S. A new spectrophotometric assay method of xanthine oxidase in crude tissue homogenate. Anal Biochem. 1974 Dec;62(2):426-35. PubMed, CrossRef
  26. Folch J, Lees M, Sloane Stanley GH. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497-509. PubMed
  27. Kates M. Techniques of lipidology. M.: Mir, 1975. 322p.
  28. Lamba OP, Borchman D, O’Brien PJ. Fourier transform infrared study of the rod outer segment disk and plasma membranes of vertebrate retina. Biochemistry. 1994 Feb 22;33(7):1704-12. PubMed, CrossRef
  29. Gavrilov VB, Gavrilova AR, Khmara NF. Measurement of diene conjugates in blood plasma using the UV absorption of heptane and isopropanol extracts. Lab Delo. 1988;(2):60-4. Russian. PubMed
  30. Stal’naya ID, Garishvili TG. Method malondialdehyde evaluation with help of tiobarbituric acid. In : Orekhovich VN, editor.  Modern methods in biochemistry. Moscow: Medicine; 1977. p. 66–7. Russian.
  31.  Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265-75. PubMed
  32. Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987 Apr;162(1):156-9. PubMed, CrossRef
  33. Konturek PC, Brzozowski T, Pierzchalski P, Kwiecien S, Pajdo R, Hahn EG, Konturek SJ. Activation of genes for spasmolytic peptide, transforming growth factor alpha and for cyclooxygenase (COX)-1 and COX-2 during gastric adaptation to aspirin damage in rats. Aliment Pharmacol Ther. 1998 Aug;12(8):767-77. PubMed, CrossRef
  34. Modlin IM, Sachs G. Inhibition of the gastric acid pump. Acid Related Diseases: Biology and Treatment. Konstanz (Germany)7 Schnetztor- Verlag GmbH D-Konstanz; 1998. p. 126-45.
  35. Shin JM, Vagin O, Munson K, Kidd M, Modlin IM, Sachs G. Molecular mechanisms in therapy of acid-related diseases. Cell Mol Life Sci. 2008 Jan;65(2):264-81. Review. PubMed, PubMedCentral, CrossRef
  36. Dröge W. Free radicals in the physiological control of cell function. Physiol Rev. 2002 Jan;82(1):47-95. Review. PubMed, CrossRef
  37. Miki H, Funato Y. Regulation of intracellular signalling through cysteine oxidation by reactive oxygen species. J Biochem. 2012 Mar;151(3):255-61. Review. PubMed, CrossRef
  38. Sakuma S, Kitamura T, Kuroda C, Takeda K, Nakano S, Hamashima T, Kohda T, Wada S, Arakawa Y, Fujimoto Y. All-trans Arachidonic acid generates reactive oxygen species via xanthine dehydrogenase/xanthine oxidase interconversion in the rat liver cytosol in vitro. J Clin Biochem Nutr. 2012 Jul;51(1):55-60. PubMed, PubMedCentral, CrossRef
  39. Romagnoli M, Gomez-Cabrera MC, Perrelli MG, Biasi F, Pallardó FV, Sastre J, Poli G, Viña J. Xanthine oxidase-induced oxidative stress causes activation of NF-kappaB and inflammation in the liver of type I diabetic rats. Free Radic Biol Med. 2010 Jul 15;49(2):171-7. PubMed, CrossRef
  40. Jones DP. Radical-free biology of oxidative stress.  Am J Physiol Cell Physiol. 2008 Oct;295(4):C849-68. PubMed, PubMedCentral, CrossRef
  41. Winczura A, Zdżalik D, Tudek B. Damage of DNA and proteins by major lipid peroxidation products in genome stability. Free Radic Res. 2012 Apr;46(4):442-59. Review. PubMed, CrossRef
  42. Smathers RL, Galligan JJ, Stewart BJ, Petersen DR. Overview of lipid peroxidation products and hepatic protein modification in alcoholic liver disease. Chem Biol Interact. 2011 Jun 30;192(1-2):107-12. Review. PubMed, PubMedCentral, CrossRef
  43. Blair IA. DNA adducts with lipid peroxidation products. J Biol Chem. 2008 Jun 6;283(23):15545-9. Review. PubMed, PubMedCentral, CrossRef
  44. Stark G. Functional consequences of oxidative membrane damage. J Membr Biol. 2005 May;205(1):1-16. Review. PubMed, CrossRef
  45. Drobinska OV, Gayda LM, Dvorshchenko KO, Tymoshenko MO, Ostapchenko LI. The state of lipid peroxidation and antioxidant protection system in parietal cells under experimental chronic atrophic gastritis development. Ukr Biokhim Zhurn. 2010 Sep-Oct;82(5):85-91. Ukrainian. PubMed
  46. Niki E. Lipid peroxidation: physiological levels and dual biological effects. Free Radic Biol Med. 2009 Sep 1;47(5):469-84. Review. PubMed, CrossRef
  47. Matsuzaki K. Modulation of TGF-beta signaling during progression of chronic liver diseases. Front Biosci (Landmark Ed). 2009 Jan 1;14:2923-34. Review. PubMed, CrossRef
  48. Black D, Lyman S, Qian T, Lemasters JJ, Rippe RA, Nitta T, Kim JS, Behrns KE. Transforming growth factor beta mediates hepatocyte apoptosis through Smad3 generation of reactive oxygen species. Biochimie. 2007 Dec;89(12):1464-73. PubMed, PubMedCentral, CrossRef
  49. Meyer C, Meindl-Beinker NM, Dooley S. TGF-beta signaling in alcohol induced hepatic injury. Front Biosci (Landmark Ed). 2010 Jan 1;15(1):740-9. Review. PubMed, CrossRef
  50. Yu Y, Lu L, Qian X, Chen N, Yao A, Pu L, Zhang F, Li X, Kong L, Sun B, Wang X. Antifibrotic effect of hepatocyte growth factor-expressing mesenchymal stem cells in small-for-size liver transplant rats. Stem Cells Dev. 2010 Jun;19(6):903-14. PubMed, CrossRef
  51. Jin SZ, Meng XW, Sun X, Han MZ, Liu BR, Wang XH, Pei FH. Hepatocyte growth factor promotes liver regeneration induced by transfusion of bone marrow mononuclear cells in a murine acute liver failure model. J Hepatobiliary Pancreat Sci. 2011 May;18(3):397-405. PubMed, CrossRef
  52. Xue F, Takahara T, Yata Y, Kuwabara Y, Shinno E, Nonome K, Minemura M, Takahara S, Li X, Yamato E, Watanabe A. Hepatocyte growth factor gene therapy accelerates regeneration in cirrhotic mouse livers after hepatectomy. Gut. 2003 May;52(5):694-700. PubMed, PubMedCentral, CrossRef
  53. Hata J, Ikeda E, Uno H, Asano S. Expression of hepatocyte growth factor mRNA in rat liver cirrhosis induced by N-nitrosodimethylamine as evidenced by in situ RT-PCR. J Histochem Cytochem. 2002 Nov;50(11):1461-8. PubMed, CrossRef
  54. Yasuda H, Imai E, Shiota A, Fujise N, Morinaga T, Higashio K. Antifibrogenic effect of a deletion variant of hepatocyte growth factor on liver fibrosis in rats. Hepatology. 1996 Sep;24(3):636-42. PubMed, CrossRef
  55. Liu RM, Gaston Pravia KA. Oxidative stress and glutathione in TGF-beta-mediated fibrogenesis. Free Radic Biol Med. 2010 Jan 1;48(1):1-15.  PubMed, PubMedCentral, CrossRef
  56. Abdulahad, S., Falalyeyeva, T., Kuharskyi V., Prybytko I., Tolstanova G., Beregova T. Study of molecular-cellular processes in health and pathology in conditions of influence of exo- and endogenous factors, biologically active drugs and substances. Visnyk Kyiv Nat Univ Taras Shevchenko. Series Problems of regulation of physiological functions. 2012;(15):4-8.
  57. Lutgendorff F, Trulsson LM, van Minnen LP, Rijkers GT, Timmerman HM, Franzén LE, Gooszen HG, Akkermans LM, Söderholm JD, Sandström PA. Probiotics enhance pancreatic glutathione biosynthesis and reduce oxidative stress in experimental acute pancreatitis. Am J Physiol Gastrointest Liver Physiol. 2008 Nov;295(5):G1111-21. PubMed, CrossRef
  58. Carroll IM, Andrus JM, Bruno-Bárcena JM, Klaenhammer TR, Hassan HM, Threadgill DS. Anti-inflammatory properties of Lactobacillus gasseri expressing manganese superoxide dismutase using the interleukin 10-deficient mouse model of colitis. Am J Physiol Gastrointest Liver Physiol. 2007 Oct;293(4):G729-38. PubMed, CrossRef

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License.